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Human Transmembrane protein 63A (TMEM63A) ELISA Kit

Human Transmembrane protein 63A (TMEM63A) ELISA Kit

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Specification

Product name Human Transmembrane protein 63A (TMEM63A) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Transmembrane protein 63A (TMEM63A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM63A in samples. An antibody specific for TMEM63A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM63A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM63A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM63A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEM63A; RP4-559A3.1; KIAA0489; KIAA0792;

Product Properties

Kit components • Human Transmembrane protein 63A microplate
• Human Transmembrane protein 63A standard
• Human Transmembrane protein 63A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Transmembrane protein 63A (TMEM63A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM63A. No significant cross-reactivity or interference between Human TMEM63A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TMEM63A is a potential multi-pass membrane protein. It belongs to the SPO75/TMEM63 family. The exact function of TMEM63A remains unknown. spo75 mutants displayed a very heterogeneous phenotype. The phenotypes of spores in spo75 cells ranged from those that had little or no spore wall material to ones that had a wild-type appearance. A transmembrane protein is a polytopic protein that spans the entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.
Gene ID 9725
Alternative TMEM63A; RP4-559A3.1; KIAA0489; KIAA0792;
Accession O94886

Image & description

Fig.1. Human Transmembrane protein 63A (TMEM63A) Standard Curve.

Fig.1. Human Transmembrane protein 63A (TMEM63A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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