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Human Transmembrane protein 173 (TMEM173) ELISA Kit

Human Transmembrane protein 173 (TMEM173) ELISA Kit

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Specification

Product name Human Transmembrane protein 173 (TMEM173) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Transmembrane protein 173 (TMEM173) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM173 in samples. An antibody specific for TMEM173 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM173 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM173 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM173 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEM173; ERIS; FLJ38577; MITA; MPYS; NET23; STING; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; endoplasmic reticulum IFN stimulator; mitochondrial mediator of IRF3 activation; st

Product Properties

Kit components • Human Transmembrane protein 173 microplate
• Human Transmembrane protein 173 standard
• Human Transmembrane protein 173 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Transmembrane protein 173 (TMEM173) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM173. No significant cross-reactivity or interference between Human TMEM173 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced 379-amino acid protein has a calculated molecular mass of 42.2 kD. It has 5 putative N-terminal transmembrane domains, a signal cleavage site in the first transmembrane domain, and a leucine-rich region that overlaps the first 4 transmembrane domains. Northern blot analysis detected STING expression in all tissues examined. Confocal microscopy and fractionation analysis of human embryonic kidney 293 cells revealed that STING predominantly associated with the endoplasmic reticulum (ER). Western blot analysis of 293 cells detected endogenous STING at an apparent molecular mass of 42 kD.STING activated both the NF-kappa-B and IRF3 transcription pathways to induce expression of IFN-alpha (IFNA1) and IFN-beta and exert a potent antiviral effect.
Gene ID 340061
Alternative TMEM173; ERIS; FLJ38577; MITA; MPYS; NET23; STING; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; endoplasmic reticulum IFN stimulator; mitochondrial mediator of IRF3 activation; st
Accession Q86WV6

Image & description

Fig.1. Human Transmembrane protein 173 (TMEM173) Standard Curve.

Fig.1. Human Transmembrane protein 173 (TMEM173) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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