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Human Transmembrane protein 158 (TMEM158) ELISA Kit

Human Transmembrane protein 158 (TMEM158) ELISA Kit

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Specification

Product name Human Transmembrane protein 158 (TMEM158) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Transmembrane protein 158 (TMEM158) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM158 in samples. An antibody specific for TMEM158 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM158 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM158 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM158 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEM158; BBP; DKFZp586E1621; RIS1; p40BBP; BINP receptor; Ras induced senescence 1; Ras-induced senescence 1; brain injury-derived neurotrophic peptide (BINP) binding protein; brain specific binding protein

Product Properties

Kit components • Human Transmembrane protein 158 microplate
• Human Transmembrane protein 158 standard
• Human Transmembrane protein 158 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Transmembrane protein 158 (TMEM158) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM158. No significant cross-reactivity or interference between Human TMEM158 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. RIS1 is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. RIS1 is not upregulated in association to any of the above-mentioned processes, but exclusively during Ras-senescence. Furthermore, RIS1 is also upregulated by the transcriptional factor Ets2, which is a known mediator of Ras-induced senescence. Interestingly, RIS1 is located at chromosomal position 3p21.3 and, more specifically, it is included in a short segment of just 1 Mb previously defined by other investigators for its tumor-suppressor activity.
Gene ID 25907
Alternative TMEM158; BBP; DKFZp586E1621; RIS1; p40BBP; BINP receptor; Ras induced senescence 1; Ras-induced senescence 1; brain injury-derived neurotrophic peptide (BINP) binding protein; brain specific binding protein
Accession Q8WZ71

Image & description

Fig.1. Human Transmembrane protein 158 (TMEM158) Standard Curve.

Fig.1. Human Transmembrane protein 158 (TMEM158) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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