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Human Transforming growth factor-beta-stimulated Protein clone-22 (TSC22) ELISA Kit

Human Transforming growth factor-beta-stimulated Protein clone-22 (TSC22) ELISA Kit

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Specification

Product name Human Transforming growth factor-beta-stimulated Protein clone-22 (TSC22) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Transforming growth factor-beta-stimulated Protein clone-22 (TSC22) ELISA Kit employs a two-site sandwich ELISA to quantitate TSC22D1 in samples. An antibody specific for TSC22D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSC22D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSC22D1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSC22D1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TSC22D1; DKFZp686O19206; MGC17597; RP11-269C23.2; TGFB1I4; TSC22; TSC22 domain family 1; isoform 2; transforming growth factor beta 1 induced transcript 4; transforming growth factor beta-stimulated protein TS

Product Properties

Kit components • Human Transforming growth factor-beta-stimulated Protein clone-22 microplate
• Human Transforming growth factor-beta-stimulated Protein clone-22 standard
• Human Transforming growth factor-beta-stimulated Protein clone-22 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Transforming growth factor-beta-stimulated Protein clone-22 (TSC22) ELISA Kit has high sensitivity and excellent specificity for detection of Human TSC22D1. No significant cross-reactivity or interference between Human TSC22D1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background domain family 1 is a member of a family of leucine zipper containing transcription factors with repressor activity and belongs to the large family of early response genes. TSC-22 regulates cell growth and differentiation, and cell death. Leucine zipper structure of TSC22 inhibits the anchorage independent growth of cancer cells. TSC-22 is also an important downstream component of peroxisome proliferator-activated receptor (PPAR) and TGF-beta signaling during intestinal epithelial cell differentiation. TSC22 encodes a transcription factor and belongs to the large family of early response genes.TSC22D1 forms homodimers via its conserved leucine zipper domain and heterodimerizes with TSC22D4. TSC22D1 has transcriptional repressor activity.
Gene ID 8848
Alternative TSC22D1; DKFZp686O19206; MGC17597; RP11-269C23.2; TGFB1I4; TSC22; TSC22 domain family 1; isoform 2; transforming growth factor beta 1 induced transcript 4; transforming growth factor beta-stimulated protein TS
Accession Q15714

Image & description

Fig.1. Human Transforming growth factor-beta-stimulated Protein clone-22 (TSC22) Standard Curve.

Fig.1. Human Transforming growth factor-beta-stimulated Protein clone-22 (TSC22) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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