| Product name | Human TRAIL Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human TRAIL Protein (Arg115-Gly281) (P50591-1) was expressed with 6×His tag at the C-terminus. |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is 10.4-15.4 ng/mL. |
| Protein length | The recombinant Human TRAIL Protein consists of 167 amino acids and predicts a molecular mass of 20.33 kDa. |
| Preparation method | E.coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Molecular Formula | 20.33 kDa |
| Alternative | TNF-related apoptosis-inducing ligand, TNFSF10, Apo2 Ligand, TL2, Apo2L, CD253 |
| Formulation | Lyophilized from sterile PBS，pH 8.0. |
| Features & Benefits | <0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 20.33 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Tumor necrosis factor‑associated apoptosis‑inducing ligand (TRAIL) is a type II membrane protein of the tumor necrosis factor (TNF) family members, moreover, expressed in many adult tissues including the thymus, prostate, colon, ovary and lung. TRAIL is a 19kDa protein containing 281 residues. TRAIL to induce apoptosis in human breast carcinoma cells (MCF7) and human epitheloid carcinoma (HeLa) cell lines, by activate two death receptors of DR4 and DR5 or two decoy receptors DcR1 and DcR2. |
| Gene ID | 8743 |
| Alternative | TNF-related apoptosis-inducing ligand, TNFSF10, Apo2 Ligand, TL2, Apo2L, CD253 |
| Accession | P50591 |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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