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Human Tomoregulin-2 (TMEFF2) ELISA Kit

Human Tomoregulin-2 (TMEFF2) ELISA Kit

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Product name Human Tomoregulin-2 (TMEFF2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tomoregulin-2 (TMEFF2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEFF2 in samples. An antibody specific for TMEFF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEFF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEFF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEFF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEFF2; HPP1; TENB2; TPEF; TR; tomoregulin; transmembrane protein TENB2

Product Properties

Kit components • Human Tomoregulin-2 microplate
• Human Tomoregulin-2 standard
• Human Tomoregulin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tomoregulin-2 (TMEFF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEFF2. No significant cross-reactivity or interference between Human TMEFF2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced 368-amino acid TRa protein contains 2 follistatin-like domains and a conserved EGF-like domain. It also has a predicted N-terminal signal peptide, a transmembrane domain, a cytosolic tail G protein-activating motif, and potential sites for N-linked glycosylation and glycosaminoglycan attachment. Unlike other EGF/NRG family members, TRa has a histidine replacing a conserved arginine essential for EGF receptor recognition. The TRb and TRc proteins contain 418 and 379 amino acids, respectively, and differ from one another and TRa in the cytoplasmic domain. Northern blot analysis detected expression of approximately 2.2- and 3.1-kb TR transcripts predominantly in brain. The 374-amino acid TMEFF2 protein shares approximately 36% and 99% sequence identity with TMEFF1 and mouse Tmeff2, respectively.
Gene ID 23671
Alternative TMEFF2; HPP1; TENB2; TPEF; TR; tomoregulin; transmembrane protein TENB2
Accession Q9UIK5

Image & description

Fig.1. Human Tomoregulin-2 (TMEFF2) Standard Curve.

Fig.1. Human Tomoregulin-2 (TMEFF2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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