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Human Tolloid-like protein 2 (TLL2) ELISA Kit

Human Tolloid-like protein 2 (TLL2) ELISA Kit

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Specification

Product name Human Tolloid-like protein 2 (TLL2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tolloid-like protein 2 (TLL2) ELISA Kit employs a two-site sandwich ELISA to quantitate TLL2 in samples. An antibody specific for TLL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TLL2; KIAA0932; MGC133312; MGC133313;

Product Properties

Kit components • Human Tolloid-like protein 2 microplate
• Human Tolloid-like protein 2 standard
• Human Tolloid-like protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tolloid-like protein 2 (TLL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLL2. No significant cross-reactivity or interference between Human TLL2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TLL2 encodes an astacin-like zinc-dependent metalloprotease and is a subfamily member of the metzincin family. Unlike other family members, a similar protein in mice does not cleave procollagen C-propeptides or chordin.TLL2 shares 80.3% amino acid sequence identity with BMP1, including 870 overlapping residues. Analysis by RT-PCR ELISA demonstrated weak expression of TLL2 in most tissues and moderate expression in heart and brain.TLL2 encodes a deduced 1,015-amino acid protein with a molecular mass of 120 kD by SDS/PAGE.Whereas BMP1, TLD, and TLL1 specifically process procollagen C-propeptides at the physiologically relevant site, TLL2 lacks this activity. BMP1 and TLL1 cleave chordin, but TLL2 and TLD do not.
Gene ID 7093
Alternative TLL2; KIAA0932; MGC133312; MGC133313;
Accession Q9Y6L7

Image & description

Fig.1. Human Tolloid-like protein 2 (TLL2) Standard Curve.

Fig.1. Human Tolloid-like protein 2 (TLL2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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