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Human Toll-like receptor 5 (TLR-5) ELISA Kit

Human Toll-like receptor 5 (TLR-5) ELISA Kit

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Specification

Product name Human Toll-like receptor 5 (TLR-5) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Toll-like receptor 5 (TLR-5) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR5 in samples. An antibody specific for TLR5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TLR5; RP11-239E10.1; FLJ10052; MGC126430; MGC126431; SLEB1; TIL3; OTTHUMP00000036039; toll/interleukin-1 receptor-like protein 3

Product Properties

Kit components • Human Toll-like receptor 5 microplate
• Human Toll-like receptor 5 standard
• Human Toll-like receptor 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Toll-like receptor 5 (TLR-5) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLR5. No significant cross-reactivity or interference between Human TLR5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Toll-like receptor 5is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene product is expressed in myelomonocytic cells, and recognizes bacterial flagellin, a principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB and stimulates tumour necrosis factor-alpha production.
Gene ID 7100
Alternative TLR5; RP11-239E10.1; FLJ10052; MGC126430; MGC126431; SLEB1; TIL3; OTTHUMP00000036039; toll/interleukin-1 receptor-like protein 3
Accession O60602

Image & description

Fig.1. Human Toll-like receptor 5 (TLR-5) Standard Curve.

Fig.1. Human Toll-like receptor 5 (TLR-5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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