| Product name | Human TNF-β Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human TNF-β (Leu 35-Leu 205) (P01374) was expressed with 6×His tag at the N-terminus. |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 10⁸ IU/mg. |
| Protein length | The recombinant Human TNF-β consists of 171 amino acids and predicts a molecular mass of 18.65 kDa. |
| Preparation method | E.coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Molecular Formula | 18.65 kDa |
| Alternative | tumor necrosis factor beta, TNFSF1B, Lymphotoxin-alpha (LT-α),LTA |
| Formulation | Lyophilized from sterile 20mM sodium citrate,0.2 M NaCl, pH 3.5. |
| Features & Benefits | <0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 18.65 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Human TNF beta (Lymphotoxin-alpha) is a 22 kDa cytokine with 205 amino acid residues. TNF beta belongs to tumor necrosis factor (TNF) superfamily as TNF alpha. Binding to lymphotoxin-β receptor (LtβR), canonical and non-canonical NF-κB signal pathway will be activated. TNF beta is related to apoptosis and inflammatory signals |
| Gene ID | 4049 |
| Alternative | tumor necrosis factor beta, TNFSF1B, Lymphotoxin-alpha (LT-α),LTA |
| Accession | P01374 |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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