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Human Thymidylate synthase (TYMS) ELISA Kit

Human Thymidylate synthase (TYMS) ELISA Kit

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Specification

Product name Human Thymidylate synthase (TYMS) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Thymidylate synthase (TYMS) ELISA Kit employs a two-site sandwich ELISA to quantitate TYMS in samples. An antibody specific for TYMS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTYMS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TYMS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TYMS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TYMS; HST422; MGC88736; TMS; TS; TSase; thymidylate synthase

Product Properties

Kit components • Human Thymidylate synthase microplate
• Human Thymidylate synthase standard
• Human Thymidylate synthase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Thymidylate synthase (TYMS) ELISA Kit has high sensitivity and excellent specificity for detection of Human TYMS. No significant cross-reactivity or interference between Human TYMS and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Thymidylate synthetase (EC 2.1.1.45) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product. As an anti-cancer chemotherapy target, thymidylate synthetase can be inhibited by the thymidylate synthase inhibitors such as fluorinated pyrimidine fluorouracil, or certain folate analogues, including most notably raltitrexed (trade name Tomudex).
Gene ID 7298
Alternative TYMS; HST422; MGC88736; TMS; TS; TSase; thymidylate synthase
Accession P04818

Image & description

Fig.1. Human Thymidylate synthase (TYMS) Standard Curve.

Fig.1. Human Thymidylate synthase (TYMS) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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