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Human Thymidinephosphorylase (TP) ELISA Kit

Human Thymidinephosphorylase (TP) ELISA Kit

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Product name Human Thymidinephosphorylase (TP) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Thymidinephosphorylase (TP) ELISA Kit employs a two-site sandwich ELISA to quantitate TYMP in samples. An antibody specific for TYMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTYMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TYMP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TYMP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TYMP; ECGF1; MNGIE; PDECGF; TP; hPD-ECGF; OTTHUMP00000196769; endothelial cell growth factor 1 (platelet-derived); gliostatin

Product Properties

Kit components • Human Thymidinephosphorylase microplate
• Human Thymidinephosphorylase standard
• Human Thymidinephosphorylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Thymidinephosphorylase (TP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TYMP. No significant cross-reactivity or interference between Human TYMP and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Platelet-derived endothelial cell growth factor is a novel angiogenic factor distinct from the previously described endothelial cell mitogens of the fibroblast growth factor family. PD-ECGF, which is stored in platelets as a 45-kD single polypeptide chain, has a highly restricted target cell specificity acting only on endothelial cells. It promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. Cloning of the gene revealed that it contains 10 exons spanning more than 4.3 kb .Using an ECGF1 cDNA probe, Stenman et al. assigned the ECGF1 gene to chromosome 22 by analysis of its segregation in a panel of human/rodent somatic cell hybrids. By in situ hybridization, they sublocalized the gene to 22q13.
Gene ID 1890
Alternative TYMP; ECGF1; MNGIE; PDECGF; TP; hPD-ECGF; OTTHUMP00000196769; endothelial cell growth factor 1 (platelet-derived); gliostatin
Accession P19971

Image & description

Fig.1. Human Thymidinephosphorylase (TP) Standard Curve.

Fig.1. Human Thymidinephosphorylase (TP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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