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Human TGF-β2 Protein, His tag (Animal-Free)

Human TGF-β2 Protein, His tag (Animal-Free)

Views(30) Publications(0) Catalog no(PRP1007)
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Specification

Product name Human TGF-β2 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human TGF-β2 (Ala 303-Ser 414) (P61812-1) was expressed with 6×His tag at the C-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells.The ED₅₀ for this effect is <0.2 ng/mL.The specific activity of recombinant human TGF beta 2 is > 5 x 10⁶ IU/mg.
Protein length The recombinant Human TGF-β2 consists of 112 amino acids and predicts a molecular mass of 13.66 kDa.
Preparation method E.coli
Purity > 98 % as determined by SDS-PAGE
Molecular Formula 13.66 kDa
Alternative G-TSF, LDS4, TGFB2

Product Properties

Formulation Lyophilized from sterile solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Features & Benefits <0.01 EU per μg of the protein as determined by the LAL method.
Molecular weight 13.66 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Transforming Growth Factors beta 2 (TGFβ-2) is a 12.85 kDa member of the epidermal Growth Factors with 113 amino acid residues. TGFβ-2 is expressed from throughout the body. TGFβ-2 is a regulator of cell proliferation, differentiation, apoptosis, cell plasticity and migration, etc. TGF-β-2 also associates with various kinds of diseases, such as cancer and tissue fibrosis.
Gene ID 7042
Alternative G-TSF, LDS4, TGFB2
Accession P61812

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