Product name | Human TGF-α protein |
Sequence | Amino acid sequence derived from Human TGF-α isoform (P01135)(Val40-Ala89) was expressed. |
Protein length | The recombinant human TGF-α consists of 50 amino acids and has a predicted molecular mass of 5.5 kDa. The apparent molecular mass of the human TGF-α protein is approximately 5.5 kDa in SDS-PAGE under reducing conditions . |
Preparation method | E. coli |
Purity | ≥ 98 % as determined by SDS-PAGE |
Formulation | Lyophilized from sterile 20 mM Tris, 50 mM NaCl pH 6.5. |
Molecular weight | 5.5 kDa |
Usage notes | Always centrifuge tubes before opening . It is recommended to centrifuge at 12000 rpm for 1 minute. Then reconstitute the lyophilized Human TGF-alpha protein with the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage instructions | Lyophilized Human TGF-α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.127% HSA or BSA). Please prevent freeze-thaw cycles. |
Shipping | The product is shipped at ambient temperature. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-α is a well-characterized mammalian growth factor that might contribute to the development of Cleft lip and palate (CL/P). |
Gene ID | 7039 |
Accession | P01135 |
Fig.SDS-PAGE analysis of Human TGF-alpha protein
Author:W Yan, M Shen, K Sun, S Li, J Miao, J Wang, J Xu Publication name:Biomedicines IF:4.7
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