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Human Mammaglobin-A (SCGB2A2) ELISA Kit

Human Mammaglobin-A (SCGB2A2) ELISA Kit

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Specification

Product name Human Mammaglobin-A (SCGB2A2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Mammaglobin-A (SCGB2A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SCGB2A2 in samples. An antibody specific for SCGB2A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCGB2A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCGB2A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCGB2A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SCGB2A2; MGB1; MGC71974; UGB2; mammaglobin 1; mammaglobin A

Product Properties

Kit components • Human Mammaglobin-A microplate
• Human Mammaglobin-A standard
• Human Mammaglobin-A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Mammaglobin-A (SCGB2A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCGB2A2. No significant cross-reactivity or interference between Human SCGB2A2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SCGB2A2 is member of the superfamily of secretoglobins, a group of small dimeric secreted and sometimes glycosylated proteins. Expressed mainly in mucosa, secretoglobins seems to be involved in cell signalling, immune response, chemotaxis, and could also serve as transporters for steroid hormones in humans. MGB1 encodes a deduced 93-amino acid protein with a 19-amino acid hydrophobic peptide signal sequence and a predicted molecular mass of 10.5-kD. The protein shares 42% sequence identity with the rat prostatein protein (rPSC3). It also shares high homology with rabbit and human uteroglobin (UGB) genes, including the conservation of cysteines known to play a role in disulfide bond formation between uteroglobin subunits and a conserved tyrosine required for progesterone binding to the uteroglobin dimer.
Gene ID 4250
Alternative SCGB2A2; MGB1; MGC71974; UGB2; mammaglobin 1; mammaglobin A
Accession Q13296

Image & description

Fig.1. Human Mammaglobin-A (SCGB2A2) Standard Curve.

Fig.1. Human Mammaglobin-A (SCGB2A2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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