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Human Tensin-3 (TNS3) ELISA Kit

Human Tensin-3 (TNS3) ELISA Kit

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Specification

Product name Human Tensin-3 (TNS3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tensin-3 (TNS3) ELISA Kit employs a two-site sandwich ELISA to quantitate TNS3 in samples. An antibody specific for TNS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNS3; DKFZp686K12123; DKFZp686M1045; FLJ13732; FLJ35545; H_NH049I23.2; MGC88434; TEM6; TENS1; H_NH0549I23.2; OTTHUMP00000082709; tensin-like SH2 domain containing 1; tensin-like SH2 domain-containing 1; thyro

Product Properties

Kit components • Human Tensin-3 microplate
• Human Tensin-3 standard
• Human Tensin-3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tensin-3 (TNS3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNS3. No significant cross-reactivity or interference between Human TNS3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tensin-3 formed a complex with focal adhesion kinase and p130Cas (BCAR1) in human breast carcinoma cells. Addition of EGF to these cells induced dephosphorylation of FAK and p130Cas, leading to dissociation of the tensin-3/FAK/p130Cas complex and enhanced interaction between tensin-3 and EGFR. The deduced 1,445-amino acid protein has a calculated molecular mass of 155.3 kD. Like other tensins, tensin-3 has an N-terminal actin-binding domain and C-terminal SH2 and PTB domains. It also has several potential tyrosine phosphorylation sites. Northern blot analysis detected high expression of an 8-kb transcript in kidney and placenta, with weaker expression in heart, skeletal muscle, spleen, liver, and lung. Western blot analysis revealed a 180-kD tensin-3 protein in human endothelial and epithelial cells and fibroblasts.
Gene ID 64759
Alternative TNS3; DKFZp686K12123; DKFZp686M1045; FLJ13732; FLJ35545; H_NH049I23.2; MGC88434; TEM6; TENS1; H_NH0549I23.2; OTTHUMP00000082709; tensin-like SH2 domain containing 1; tensin-like SH2 domain-containing 1; thyro
Accession Q68CZ2

Image & description

Fig.1. Human Tensin-3 (TNS3) Standard Curve.

Fig.1. Human Tensin-3 (TNS3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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