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Human Tenascin-R (TNR) ELISA Kit

Human Tenascin-R (TNR) ELISA Kit

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Specification

Product name Human Tenascin-R (TNR) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tenascin-R (TNR) ELISA Kit employs a two-site sandwich ELISA to quantitate TNR in samples. An antibody specific for TNR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNR; RP3-518E13.1; MGC149328; TN-R; OTTHUMP00000032802; tenascin R

Product Properties

Kit components • Human Tenascin-R microplate
• Human Tenascin-R standard
• Human Tenascin-R detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tenascin-R (TNR) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNR. No significant cross-reactivity or interference between Human TNR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tenascins are extracellular matrix glycoproteins. They are abundant in the extracellular matrix of developing vertebrate embryos and they reappear around healing wounds and in the stroma of some tumors.Tenascin-R is a tenascin gene.Tenascin-R is found in the developing and adult nervous system. Tenascin-R (TNR) is an extracellular matix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion), TNX (TNXB), and TNR (Erickson, 1993). The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues.
Gene ID 7143
Alternative TNR; RP3-518E13.1; MGC149328; TN-R; OTTHUMP00000032802; tenascin R
Accession Q92752

Image & description

Fig.1. Human Tenascin-R (TNR) Standard Curve.

Fig.1. Human Tenascin-R (TNR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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