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Human Tankyrase-1 (TNKS) ELISA Kit

Human Tankyrase-1 (TNKS) ELISA Kit

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Specification

Product name Human Tankyrase-1 (TNKS) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Tankyrase-1 (TNKS) ELISA Kit employs a two-site sandwich ELISA to quantitate TNKS in samples. An antibody specific for TNKS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNKS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNKS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNKS; PARP-5a; PARP5A; PARPL; TIN1; TINF1; TNKS1;

Product Properties

Kit components • Human Tankyrase-1 microplate
• Human Tankyrase-1 standard
• Human Tankyrase-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Tankyrase-1 (TNKS) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNKS. No significant cross-reactivity or interference between Human TNKS and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tankyrase 1, a human telomeric poly(ADP-ribose) polymerase, was originally identified through its interaction with TRF1, a negative regulator of telomere length. Tankyrase 1 ADP-ribosylates TRF1 in vitro, and its overexpression induces telomere elongation in human cancer cells. Surprisingly, dissection of this domain reveals multiple discrete and overlapping binding sites for TRF1 and TAB182. Five well conserved ankyrin repeat clusters in tankyrase 1. Although each of the five ankyrin repeat clusters independently binds to TRF1, only three of the five bind toTAB182. These findings suggest that tankyrase 1 may act as a scaffold for large molecular mass complexes made up of multiple binding proteins. Potential roles for tankyrase 1-mediated higher order complexes at telomeres and at other subcellular sites.
Gene ID 8658
Alternative TNKS; PARP-5a; PARP5A; PARPL; TIN1; TINF1; TNKS1;
Accession O95271

Image & description

Fig.1. Human Tankyrase-1 (TNKS) Standard Curve.

Fig.1. Human Tankyrase-1 (TNKS) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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