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Human T-cell acute lymphocytic leukemia protein 1 (TAL1) ELISA Kit

Human T-cell acute lymphocytic leukemia protein 1 (TAL1) ELISA Kit

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Specification

Product name Human T-cell acute lymphocytic leukemia protein 1 (TAL1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human T-cell acute lymphocytic leukemia protein 1 (TAL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAL1 in samples. An antibody specific for TAL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TAL1; SCL; TCL5; bHLHa17; tal-1; OTTHUMP00000009563; tal-1 product

Product Properties

Kit components • Human T-cell acute lymphocytic leukemia protein 1 microplate
• Human T-cell acute lymphocytic leukemia protein 1 standard
• Human T-cell acute lymphocytic leukemia protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human T-cell acute lymphocytic leukemia protein 1 (TAL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAL1. No significant cross-reactivity or interference between Human TAL1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TAL1 (TAL BHLH Transcription Factor 1, Erythroid Differentiation Factor) is a Protein Coding gene. Diseases associated with TAL1 include Leukemia, Acute Lymphoblastic and Precursor T-Cell Acute Lymphoblastic Leukemia. Among its related pathways are MAPK-Erk Pathway and NF-kappaB Signaling. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and chromatin binding. An important paralog of this gene is LYL1. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.
Gene ID 6886
Alternative TAL1; SCL; TCL5; bHLHa17; tal-1; OTTHUMP00000009563; tal-1 product
Accession P17542

Image & description

Fig.1. Human T-cell acute lymphocytic leukemia protein 1 (TAL1) Standard Curve.

Fig.1. Human T-cell acute lymphocytic leukemia protein 1 (TAL1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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