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Human Synaptotagmin-like protein 2 (SYTL2) ELISA Kit

Human Synaptotagmin-like protein 2 (SYTL2) ELISA Kit

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Specification

Product name Human Synaptotagmin-like protein 2 (SYTL2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Synaptotagmin-like protein 2 (SYTL2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYTL2 in samples. An antibody specific for SYTL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYTL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYTL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYTL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SYTL2; CHR11SYT; EXO4; FLJ21219; FLJ34802; FLJ42764; FLJ45597; KIAA1597; MGC102768; SGA72M; SLP2; SLP2A; breast cancer-associated antigen SGA-72M; chromosome 11 synaptotagmin; exophilin-4

Product Properties

Kit components • Human Synaptotagmin-like protein 2 microplate
• Human Synaptotagmin-like protein 2 standard
• Human Synaptotagmin-like protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Synaptotagmin-like protein 2 (SYTL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYTL2. No significant cross-reactivity or interference between Human SYTL2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Synaptotagmin-like protein 2 (SYTL2) is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of SYTL2 has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). SYTL2 plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID 54843
Alternative SYTL2; CHR11SYT; EXO4; FLJ21219; FLJ34802; FLJ42764; FLJ45597; KIAA1597; MGC102768; SGA72M; SLP2; SLP2A; breast cancer-associated antigen SGA-72M; chromosome 11 synaptotagmin; exophilin-4
Accession Q9HCH5

Image & description

Fig.1. Human Synaptotagmin-like protein 2 (SYTL2) Standard Curve.

Fig.1. Human Synaptotagmin-like protein 2 (SYTL2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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