|Product name||Human Synaptotagmin-13 (SYT13) ELISA Kit|
|Applications notes||This Human Synaptotagmin-13 (SYT13) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT13 in samples. An antibody specific for SYT13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT13 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Synaptotagmin-13 microplate
• Human Synaptotagmin-13 standard
• Human Synaptotagmin-13 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Synaptotagmin-13 (SYT13) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT13. No significant cross-reactivity or interference between Human SYT13 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Unlike most of the synaptotagmins, SYT13 does not have an N-terminal transmembrane region. Its C2 domains are lacking almost all the residues involved in Ca2+ binding. It is highly expressed in brain and also detectable at lower levels in non-neuronal tissues. SYT13 can suppress liver tumour cells and this function may be mediated through pathways implicated in mesenchymal to epithelial transition. It also affects insulin secretion.|
Fig.1. Human Synaptotagmin-13 (SYT13) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.