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Human Surfactant-associated protein 2 (SFTA2) ELISA Kit

Human Surfactant-associated protein 2 (SFTA2) ELISA Kit

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Specification

Product name Human Surfactant-associated protein 2 (SFTA2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Surfactant-associated protein 2 (SFTA2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTA2 in samples. An antibody specific for SFTA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SFTA2; DADB-160J14.6; GSGL541; SFTPG; UNQ541; surfactant associated protein G

Product Properties

Kit components • Human Surfactant-associated protein 2 microplate
• Human Surfactant-associated protein 2 standard
• Human Surfactant-associated protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Surfactant-associated protein 2 (SFTA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFTA2. No significant cross-reactivity or interference between Human SFTA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SFTPGis an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period.
Gene ID 389376
Alternative SFTA2; DADB-160J14.6; GSGL541; SFTPG; UNQ541; surfactant associated protein G
Accession Q6UW10

Image & description

Fig.1. Human Surfactant-associated protein 2 (SFTA2) Standard Curve.

Fig.1. Human Surfactant-associated protein 2 (SFTA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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