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Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) ELISA Kit

Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) ELISA Kit

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Specification

Product name Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) ELISA Kit employs a two-site sandwich ELISA to quantitate STK39 in samples. An antibody specific for STK39 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTK39 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STK39 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STK39 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative STK39; DCHT; DKFZp686K05124; PASK; SPAK; Ste20-like protein kinase; proline-alanine-rich STE20-related kinase; serine/threonine kinase 39; small intestine SPAK-like kinase

Product Properties

Kit components • Human STE20/SPS1-related proline-alanine-rich protein kinase microplate
• Human STE20/SPS1-related proline-alanine-rich protein kinase standard
• Human STE20/SPS1-related proline-alanine-rich protein kinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) ELISA Kit has high sensitivity and excellent specificity for detection of Human STK39. No significant cross-reactivity or interference between Human STK39 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The deduced 547-amino acid protein has a calculated molecular mass of 59.6 kD. STK39 contains an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detected ubiquitous expression of a 3.8-kb transcript, with most abundant expression in brain and pancreas. Western blot analysis of mouse tissues detected a protein of about 60 kD in all tissues examined.
Gene ID 27347
Alternative STK39; DCHT; DKFZp686K05124; PASK; SPAK; Ste20-like protein kinase; proline-alanine-rich STE20-related kinase; serine/threonine kinase 39; small intestine SPAK-like kinase
Accession Q9UEW8

Image & description

Fig.1. Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) Standard Curve.

Fig.1. Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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