| Product name | Human STE20-like serine/threonine-protein kinase (SLK) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human STE20-like serine/threonine-protein kinase (SLK) ELISA Kit employs a two-site sandwich ELISA to quantitate SLK in samples. An antibody specific for SLK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLK bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SLK; KIAA0204; LOSK; MGC133067; STK2; bA16H23.1; se20-9; CTCL tumor antigen se20-9; Long Ste20-like Kinase; SNF1 (sucrose nonfermenting; yeast; homolog)-like kinase; SNF1 sucrose nonfermenting like kinase; |
| Kit components | • Human STE20-like serine/threonine-protein kinase microplate • Human STE20-like serine/threonine-protein kinase standard • Human STE20-like serine/threonine-protein kinase detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human STE20-like serine/threonine-protein kinase (SLK) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLK. No significant cross-reactivity or interference between Human SLK and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | FYN encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. Fyn is a tyrosine specific phospho-transferase that is a member of the Src family of tyrosine protein kinases.Fyn is primarily localized to the cytoplasmic leaflet of the plasma membrane, where it phosphorylates tyrosine residues on key targets involved in a variety of different signaling pathways. Tyrosine phosphorylation of target proteins by Fyn serves to either regulate target protein activity, and/or to generate a binding site on the target protein that recruits other signaling molecules. |
| Gene ID | 9748 |
| Alternative | SLK; KIAA0204; LOSK; MGC133067; STK2; bA16H23.1; se20-9; CTCL tumor antigen se20-9; Long Ste20-like Kinase; SNF1 (sucrose nonfermenting; yeast; homolog)-like kinase; SNF1 sucrose nonfermenting like kinase; |
| Accession | Q9H2G2 |
Fig.1. Human STE20-like serine/threonine-protein kinase (SLK) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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