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Human Sphingosine kinase 2 (SPHK2) ELISA Kit

Human Sphingosine kinase 2 (SPHK2) ELISA Kit

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Product name Human Sphingosine kinase 2 (SPHK2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Sphingosine kinase 2 (SPHK2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPHK2 in samples. An antibody specific for SPHK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPHK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPHK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPHK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPHK2; Sphingosine kinase type 2 isoform

Product Properties

Kit components • Human Sphingosine kinase 2 microplate
• Human Sphingosine kinase 2 standard
• Human Sphingosine kinase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Sphingosine kinase 2 (SPHK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPHK2. No significant cross-reactivity or interference between Human SPHK2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Sphingosine kinase 2 (SPHK2) is one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. SPHK2 may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for SPHK2.
Gene ID 56848
Alternative SPHK2; Sphingosine kinase type 2 isoform
Accession Q9NRA0

Image & description

Fig.1. Human Sphingosine kinase 2 (SPHK2) Standard Curve.

Fig.1. Human Sphingosine kinase 2 (SPHK2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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