| Product name | Human Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit employs a two-site sandwich ELISA to quantitate SORBS2 in samples. An antibody specific for SORBS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySORBS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORBS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORBS2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SORBS2; ARGBP2; FLJ93447; KIAA0777; PRO0618; Arg binding protein 2; Arg/Abl interacting protein; Arg/Abl-interacting protein 2; OTTHUMP00000211726 |
| Kit components | • Human Sorbin and SH3 domain-containing protein 2 microplate • Human Sorbin and SH3 domain-containing protein 2 standard • Human Sorbin and SH3 domain-containing protein 2 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SORBS2. No significant cross-reactivity or interference between Human SORBS2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. SORBS2 encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Gene ID | 8470 |
| Alternative | SORBS2; ARGBP2; FLJ93447; KIAA0777; PRO0618; Arg binding protein 2; Arg/Abl interacting protein; Arg/Abl-interacting protein 2; OTTHUMP00000211726 |
| Accession | O94875 |
Fig.1. Human Sorbin and SH3 domain-containing protein 2 (SORBS2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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