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Human Soluble tumor necrosis factor receptor 2 (sTNF-R2) ELISA Kit

Human Soluble tumor necrosis factor receptor 2 (sTNF-R2) ELISA Kit

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Specification

Product name Human Soluble tumor necrosis factor receptor 2 (sTNF-R2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Soluble tumor necrosis factor receptor 2 (sTNF-R2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF1B in samples. An antibody specific for TNFRSF1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNFRSF1B; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; p75TNFR; p75 TNF receptor; soluble TNFR1B variant 1; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; tu

Product Properties

Kit components • Human Soluble tumor necrosis factor receptor 2 microplate
• Human Soluble tumor necrosis factor receptor 2 standard
• Human Soluble tumor necrosis factor receptor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Soluble tumor necrosis factor receptor 2 (sTNF-R2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFRSF1B. No significant cross-reactivity or interference between Human TNFRSF1B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Soluble tumor necrosis factor receptor 2 (sTNF-R2) is a member of the TNF-receptor superfamily. sTNF-R2 and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.
Gene ID 7133
Alternative TNFRSF1B; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; p75TNFR; p75 TNF receptor; soluble TNFR1B variant 1; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; tu
Accession P20333

Image & description

Fig.1. Human Soluble tumor necrosis factor receptor 2 (sTNF-R2) Standard Curve.

Fig.1. Human Soluble tumor necrosis factor receptor 2 (sTNF-R2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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