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Human Sodium-dependent neutral amino acid transporter B (SLC6A19) ELISA Kit

Human Sodium-dependent neutral amino acid transporter B (SLC6A19) ELISA Kit

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Specification

Product name Human Sodium-dependent neutral amino acid transporter B (SLC6A19) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Sodium-dependent neutral amino acid transporter B (SLC6A19) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A19 in samples. An antibody specific for SLC6A19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC6A19; B0AT1; FLJ20680; FLJ34635; HND; sodium-dependent amino acid transporter system B0; solute carrier family 6 (neurotransmitter transporter); member 19; solute carrier family 6; member 19; system B0 neutr

Product Properties

Kit components • Human Sodium-dependent neutral amino acid transporter B microplate
• Human Sodium-dependent neutral amino acid transporter B standard
• Human Sodium-dependent neutral amino acid transporter B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Sodium-dependent neutral amino acid transporter B (SLC6A19) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC6A19. No significant cross-reactivity or interference between Human SLC6A19 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SLC6A19 is a system B(0) transporter that mediates epithelial resorption of neutral amino acids across the apical membrane in the kidney and intestine.The deduced 634-amino acid protein contains 12 transmembrane domains. In situ hybridization detected Slc6a19 expression in renal cortical proximal tubules and in small intestine villus enterocytes, with highest expression in apical cells. EST database analysis suggested expression in several other tissues and cells. contains 634 amino acids and has 12 predicted transmembrane regions. In a panel of human cDNAs, they detected robust expression of SLC6A19 in kidney and small intestine, with minimal expression in pancreas. SLC6A19 was also expressed in stomach, liver, duodenum, and ileocecum.
Gene ID 340024
Alternative SLC6A19; B0AT1; FLJ20680; FLJ34635; HND; sodium-dependent amino acid transporter system B0; solute carrier family 6 (neurotransmitter transporter); member 19; solute carrier family 6; member 19; system B0 neutr
Accession Q695T7

Image & description

Fig.1. Human Sodium-dependent neutral amino acid transporter B (SLC6A19) Standard Curve.

Fig.1. Human Sodium-dependent neutral amino acid transporter B (SLC6A19) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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