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Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit

Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit

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Specification

Product name Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC10A1 in samples. An antibody specific for SLC10A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC10A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC10A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC10A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC10A1; NTCP; Na/taurocholate cotransporting polypeptide; growth-inhibiting protein 29; sodium/bile acid cotransporter; sodium/taurocholate cotransporter; solute carrier family 10; member 1

Product Properties

Kit components • Human Sodium/bile acid cotransporter microplate
• Human Sodium/bile acid cotransporter standard
• Human Sodium/bile acid cotransporter detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC10A1. No significant cross-reactivity or interference between Human SLC10A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Sodium/bile acid cotransporters are integral membrane glycoproteins. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization (SLC10A2;), and the other being found in the basolateral membranes of hepatocytes (SLC10A1). SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Alanine insertion experiments confirmed that 7 of the 9 hydrophobic stretches are membrane-integrated, with secondary structures and transport activity sensitive to positional displacement. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane.
Gene ID 6554
Alternative SLC10A1; NTCP; Na/taurocholate cotransporting polypeptide; growth-inhibiting protein 29; sodium/bile acid cotransporter; sodium/taurocholate cotransporter; solute carrier family 10; member 1
Accession Q14973

Image & description

Fig.1. Human Sodium/bile acid cotransporter (SLC10A1) Standard Curve.

Fig.1. Human Sodium/bile acid cotransporter (SLC10A1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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