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Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit

Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit

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Specification

Product name Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit employs a two-site sandwich ELISA to quantitate STAT4 in samples. An antibody specific for STAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTAT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STAT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative STAT4; SLEB11; OTTHUMP00000163553

Product Properties

Kit components • Human Signal transducer and activator of transcription 4 microplate
• Human Signal transducer and activator of transcription 4 standard
• Human Signal transducer and activator of transcription 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit has high sensitivity and excellent specificity for detection of Human STAT4. No significant cross-reactivity or interference between Human STAT4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background STAT4 is phosphorylated in response to interleukin-12 and is essential for IL12 signal transduction. For further information on STATs. STAT4 and STAT1, which both map to chromosome 2q32, may have arisen via a tandem gene duplication. However, STAT1 was expressed ubiquitously, whereas STAT4 was expressed in specific tissues, including spleen, heart, brain, peripheral blood cells, and testis. STAT4 expression was drastically increased in T cells following treatment with a DNA methyltransferase inhibitor. Truncation of methylation sites in the proximal regulatory elements of the STAT4 promoter markedly enhanced transcriptional activity. The N-terminal protein interaction domain (N domain) of STAT4 is required for STAT4 activation after IL12 signaling.
Gene ID 6775
Alternative STAT4; SLEB11; OTTHUMP00000163553
Accession Q14765

Image & description

Fig.1. Human Signal transducer and activator of transcription 4 (STAT4) Standard Curve.

Fig.1. Human Signal transducer and activator of transcription 4 (STAT4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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