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Human SHC-transforming protein 1 (SHC1) ELISA Kit

Human SHC-transforming protein 1 (SHC1) ELISA Kit

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Specification

Product name Human SHC-transforming protein 1 (SHC1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human SHC-transforming protein 1 (SHC1) ELISA Kit employs a two-site sandwich ELISA to quantitate SHC1 in samples. An antibody specific for SHC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySHC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SHC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SHC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SHC1; RP11-307C12.1; FLJ26504; SHC; SHCA; OTTHUMP00000035472; SHC (Src homology 2 domain-containing) transforming protein 1; SHC-transforming protein 1

Product Properties

Kit components • Human SHC-transforming protein 1 microplate
• Human SHC-transforming protein 1 standard
• Human SHC-transforming protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human SHC-transforming protein 1 (SHC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SHC1. No significant cross-reactivity or interference between Human SHC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The SHC gene encodes a signaling and transforming protein containing Src homology 2 and 3 (SH2 and SH3) domains. The SHC gene encodes 2 widely expressed overlapping proteins of 46 and 52 kD, both containing a C-terminal SH2 domain. Adjacent to the SH2 region is a glycine- and proline-rich region. The 2 proteins differ in their N terminals. SHC proteins are involved in mitogenic signal transduction and act by coupling growth factor receptors to the RAS signaling pathway. The protein encoded by the SHC1 gene is thought to act as an adaptor in many signal transduction pathways, for example, facilitating the activation of RAS proteins in response to a variety of factors. SHC proteins are rapidly associated with and phosphorylated by growth factor receptors with intrinsic tyrosine kinase activity.
Gene ID 6464
Alternative SHC1; RP11-307C12.1; FLJ26504; SHC; SHCA; OTTHUMP00000035472; SHC (Src homology 2 domain-containing) transforming protein 1; SHC-transforming protein 1
Accession P29353

Image & description

Fig.1. Human SHC-transforming protein 1 (SHC1) Standard Curve.

Fig.1. Human SHC-transforming protein 1 (SHC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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