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Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit

Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit

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Specification

Product name Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate RIOK1 in samples. An antibody specific for RIOK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRIOK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIOK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIOK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RIOK1; AD034; FLJ30006; MGC12903; bA288G3.1; RIO kinase 1; prematurely truncated due to insertion of intronic sequence

Product Properties

Kit components • Human Serine/threonine-protein kinase RIO1 microplate
• Human Serine/threonine-protein kinase RIO1 standard
• Human Serine/threonine-protein kinase RIO1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RIOK1. No significant cross-reactivity or interference between Human RIOK1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RIOK1 includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. RioK1 and pICln bind to PRMT5 in a mutually exclusive fashion. This results in a PRMT5-WD45/MEP50 core structure that either associates with pICln or RioK1 in distinct complexes. RioK1 functions in analogy to pICln as an adapter protein by recruiting the RNA-binding protein nucleolin to the PRMT5 complex for its symmetrical methylation. The exclusive interaction of PRMT5 with either pICln or RioK1 thus provides the first mechanistic insight into how a methyltransferase can distinguish between its substrate proteins.
Gene ID 83732
Alternative RIOK1; AD034; FLJ30006; MGC12903; bA288G3.1; RIO kinase 1; prematurely truncated due to insertion of intronic sequence
Accession Q9BRS2

Image & description

Fig.1. Human Serine/threonine-protein kinase RIO1 (RIOK1) Standard Curve.

Fig.1. Human Serine/threonine-protein kinase RIO1 (RIOK1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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