| Product name | Human Semaphorin-4B (SEMA4B) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Semaphorin-4B (SEMA4B) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA4B in samples. An antibody specific for SEMA4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4B bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SEMA4B; KIAA1745; MGC131831; SEMAC; SemC; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; 4B; semaphorin 4B |
| Kit components | • Human Semaphorin-4B microplate • Human Semaphorin-4B standard • Human Semaphorin-4B detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Semaphorin-4B (SEMA4B) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA4B. No significant cross-reactivity or interference between Human SEMA4B and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Semaphorin-4B is a protein encoded by the SEMA4B gene. Members of the collapsin/semaphorin gene family have been proposed to act as growth cone guidance signals in vertebrates and invertebrates. To identify candidate molecules involved in axonal pathfinding during mouse embryogenesis, isolated cDNAs encoding five new members of the semaphorin family (Sem A-Sem E). The murine semaphorin genes are differentially expressed in mesoderm and neuroectoderm before and during the time when axons select their pathways in the embryo. In explant cultures, recombinant Sem D/collapsin converts a matrix permissive for axonal growth into one that is inhibitory for neurites of peripheral ganglia. Semaphorins are a diverse family of molecules that may provide local signals to specify territories nonaccessible for growing axons. |
| Gene ID | 10509 |
| Alternative | SEMA4B; KIAA1745; MGC131831; SEMAC; SemC; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; 4B; semaphorin 4B |
| Accession | Q9NPR2 |
Fig.1. Human Semaphorin-4B (SEMA4B) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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