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Human Semaphorin-4B (SEMA4B) ELISA Kit

Human Semaphorin-4B (SEMA4B) ELISA Kit

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Specification

Product name Human Semaphorin-4B (SEMA4B) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Semaphorin-4B (SEMA4B) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA4B in samples. An antibody specific for SEMA4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SEMA4B; KIAA1745; MGC131831; SEMAC; SemC; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; 4B; semaphorin 4B

Product Properties

Kit components • Human Semaphorin-4B microplate
• Human Semaphorin-4B standard
• Human Semaphorin-4B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Semaphorin-4B (SEMA4B) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA4B. No significant cross-reactivity or interference between Human SEMA4B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Semaphorin-4B is a protein encoded by the SEMA4B gene. Members of the collapsin/semaphorin gene family have been proposed to act as growth cone guidance signals in vertebrates and invertebrates. To identify candidate molecules involved in axonal pathfinding during mouse embryogenesis, isolated cDNAs encoding five new members of the semaphorin family (Sem A-Sem E). The murine semaphorin genes are differentially expressed in mesoderm and neuroectoderm before and during the time when axons select their pathways in the embryo. In explant cultures, recombinant Sem D/collapsin converts a matrix permissive for axonal growth into one that is inhibitory for neurites of peripheral ganglia. Semaphorins are a diverse family of molecules that may provide local signals to specify territories nonaccessible for growing axons.
Gene ID 10509
Alternative SEMA4B; KIAA1745; MGC131831; SEMAC; SemC; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; 4B; semaphorin 4B
Accession Q9NPR2

Image & description

Fig.1. Human Semaphorin-4B (SEMA4B) Standard Curve.

Fig.1. Human Semaphorin-4B (SEMA4B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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