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Human Semaphorin-3B (SEMA3B) ELISA Kit

Human Semaphorin-3B (SEMA3B) ELISA Kit

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Specification

Product name Human Semaphorin-3B (SEMA3B) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Semaphorin-3B (SEMA3B) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3B in samples. An antibody specific for SEMA3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SEMA3B; FLJ34863; LUCA-1; SEMA5; SEMAA; SemA; semaV; semaphorin 3B; semaphorin A; semaphorin V

Product Properties

Kit components • Human Semaphorin-3B microplate
• Human Semaphorin-3B standard
• Human Semaphorin-3B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Semaphorin-3B (SEMA3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA3B. No significant cross-reactivity or interference between Human SEMA3B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis. The 2 genes have widespread but distinct patterns of expression in nonneural tissues, and have different patterns of expression in lung cancer. Human semaphorin A(V) has 86% amino acid homology with murine semaphorin A, whereas semaphorin IV is more closely related to murine semaphorin E, with 50% homology.In particular, overlapping homozygous deletions in lung cancers have been identified in region 3p21.3; more than 90% of small cell lung cancers exhibit LOH at this site.
Gene ID 7869
Alternative SEMA3B; FLJ34863; LUCA-1; SEMA5; SEMAA; SemA; semaV; semaphorin 3B; semaphorin A; semaphorin V
Accession Q13214

Image & description

Fig.1. Human Semaphorin-3B (SEMA3B) Standard Curve.

Fig.1. Human Semaphorin-3B (SEMA3B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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