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Human Secreted Ly-6/uPAR-related protein 1 (SLURP1) ELISA Kit

Human Secreted Ly-6/uPAR-related protein 1 (SLURP1) ELISA Kit

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Specification

Product name Human Secreted Ly-6/uPAR-related protein 1 (SLURP1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Secreted Ly-6/uPAR-related protein 1 (SLURP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLURP1 in samples. An antibody specific for SLURP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLURP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLURP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLURP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLURP1; ANUP; ARS; ArsB; LY6LS; MDM; ARS component B; ARS(component B)-81/S; anti-neoplastic urinary protein; lymphocyte antigen 6-like secreted; secreted Ly6/uPAR related protein 1

Product Properties

Kit components • Human Secreted Ly-6/uPAR-related protein 1 microplate
• Human Secreted Ly-6/uPAR-related protein 1 standard
• Human Secreted Ly-6/uPAR-related protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Secreted Ly-6/uPAR-related protein 1 (SLURP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLURP1. No significant cross-reactivity or interference between Human SLURP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SLURP1 is a member of the Ly6/uPAR family but lacks a GPI-anchoring signal sequence. It is thought that this secreted protein contains antitumor activity. Mutations in this gene have been associated with Mal de Meleda, a rare autosomal recessive skin disorder. This gene maps to the same chromosomal region as several members of the Ly6/uPAR family of glycoprotein receptors. The Ly6/uPAR protein family can be divided into 2 subfamilies. One comprises glycosylphosphatidylinositol (GPI)-anchored glycoprotein receptors with 10 cysteine residues. The other subfamily includes the secreted single-domain snake and frog cytotoxins, and its members generally possess only 8 cysteines and no GPI-anchoring signal sequence.
Gene ID 57152
Alternative SLURP1; ANUP; ARS; ArsB; LY6LS; MDM; ARS component B; ARS(component B)-81/S; anti-neoplastic urinary protein; lymphocyte antigen 6-like secreted; secreted Ly6/uPAR related protein 1
Accession P55000

Image & description

Fig.1. Human Secreted Ly-6/uPAR-related protein 1 (SLURP1) Standard Curve.

Fig.1. Human Secreted Ly-6/uPAR-related protein 1 (SLURP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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