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Human Secretagogin (SCGN) ELISA Kit

Human Secretagogin (SCGN) ELISA Kit

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Specification

Product name Human Secretagogin (SCGN) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Secretagogin (SCGN) ELISA Kit employs a two-site sandwich ELISA to quantitate SCGN in samples. An antibody specific for SCGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCGN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCGN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SCGN; CALBL; DJ501N12.8; SECRET; SEGN; setagin; calbindin-like; secretagogin

Product Properties

Kit components • Human Secretagogin microplate
• Human Secretagogin standard
• Human Secretagogin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Secretagogin (SCGN) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCGN. No significant cross-reactivity or interference between Human SCGN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SCGN contains 6 tandem repeats of the EF-hand Ca(2+)-binding domain, with a loop at all 6 putative Ca(2+)-binding sites, and shares significant similarity with calbindin and calretinin. Much lower expression of only the smaller transcript was detected in adrenal medulla, stomach, thyroid, colon, and small intestine. Immunohistochemical analysis showed SCGN expression restricted to the islet of Langerhans in pancreas, in C cells near thyroid follicles, and at sites typically occupied by neuroendocrine cells in all other tissues examined. Cell fractionation and confocal imaging of transfected rat insulinoma cells detected Scgn predominantly in the cytosolic fraction, with much less Scgn in nuclei. SCGN was expressed in a reticular pattern in transfected COS cells.
Gene ID 10590
Alternative SCGN; CALBL; DJ501N12.8; SECRET; SEGN; setagin; calbindin-like; secretagogin
Accession O76038

Image & description

Fig.1. Human Secretagogin (SCGN) Standard Curve.

Fig.1. Human Secretagogin (SCGN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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