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Human S100 calcium binding protein A12/Calgranulin-C (S100A12) ELISA Kit

Human S100 calcium binding protein A12/Calgranulin-C (S100A12) ELISA Kit

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Specification

Product name Human S100 calcium binding protein A12/Calgranulin-C (S100A12) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human S100 calcium binding protein A12/Calgranulin-C (S100A12) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A12 in samples. An antibody specific for S100A12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative S100A12; CAAF1; CAGC; CGRP; ENRAGE; MRP6; p6; S100 calcium-binding protein A12; S100 calcium-binding protein A12 (calgranulin C); calgranulin C

Product Properties

Kit components • Human S100 calcium binding protein A12/Calgranulin-C microplate
• Human S100 calcium binding protein A12/Calgranulin-C standard
• Human S100 calcium binding protein A12/Calgranulin-C detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human S100 calcium binding protein A12/Calgranulin-C (S100A12) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A12. No significant cross-reactivity or interference between Human S100A12 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background S100 calcium binding protein A12 encoded by S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100 calcium binding protein A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. S100 calcium binding protein A12 includes an antimicrobial peptide which has antibacterial activity.
Gene ID 6283
Alternative S100A12; CAAF1; CAGC; CGRP; ENRAGE; MRP6; p6; S100 calcium-binding protein A12; S100 calcium-binding protein A12 (calgranulin C); calgranulin C
Accession P80511

Image & description

Fig.1. Human S100 calcium binding protein A12/Calgranulin-C (S100A12) Standard Curve.

Fig.1. Human S100 calcium binding protein A12/Calgranulin-C (S100A12) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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