| Product name | Human Runt-related transcription factor 3 (RUNX3) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Runt-related transcription factor 3 (RUNX3) ELISA Kit employs a two-site sandwich ELISA to quantitate RUNX3 in samples. An antibody specific for RUNX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRUNX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RUNX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | RUNX3; RP3-398I9.1; AML2; CBFA3; FLJ34510; MGC16070; PEBP2aC; OTTHUMP00000003371; PEA2 alpha C; PEBP2 alpha C; acute myeloid leukemia gene 2; core-binding factor; runt domain; alpha subunit 3; polyomavirus enha |
| Kit components | • Human Runt-related transcription factor 3 microplate • Human Runt-related transcription factor 3 standard • Human Runt-related transcription factor 3 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Runt-related transcription factor 3 (RUNX3) ELISA Kit has high sensitivity and excellent specificity for detection of Human RUNX3. No significant cross-reactivity or interference between Human RUNX3 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | RUNX3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. Runx3 mouse gastric mucosa exhibits hyperplasia due to stimulated proliferation and suppressed apoptosis in epithelial cells, and the cells are resistant to TGF-beta stimulation. |
| Gene ID | 864 |
| Alternative | RUNX3; RP3-398I9.1; AML2; CBFA3; FLJ34510; MGC16070; PEBP2aC; OTTHUMP00000003371; PEA2 alpha C; PEBP2 alpha C; acute myeloid leukemia gene 2; core-binding factor; runt domain; alpha subunit 3; polyomavirus enha |
| Accession | Q13761 |
Fig.1. Human Runt-related transcription factor 3 (RUNX3) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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