| Product name | Human RSPO1/R-spondin 1 Protein, His-SUMO tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human RSPO1/R-spondin 1 (Ser21-Ala263) (Q2MKA7) was expressed with His-SUMO tag at the N-terminus. |
| Applications | His-SUMO tag |
| Applications notes | Human |
| Activity | R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is 1-3 μg/mL. |
| Protein length | The recombinant Human RSPO1/R-spondin 1 consists of 243 amino acids and predicts a molecular mass of 46.8 kDa. |
| Preparation method | HEK 293 |
| Purity | > 95 % as determined by SDS-PAGE |
| Molecular Formula | 46.8 kDa |
| Alternative | RSPO1, Roof plate-specific spondin-1 |
| Formulation | Lyophilized from sterile PBS，pH 7.4. |
| Features & Benefits | <1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 46.8 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis. |
| Gene ID | 284654 |
| Alternative | RSPO1, Roof plate-specific spondin-1 |
| Accession | Q2MKA7 |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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