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Human RRNA methyltransferase 1, mitochondrial (MRM1) ELISA Kit

Human RRNA methyltransferase 1, mitochondrial (MRM1) ELISA Kit

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Specification

Product name Human RRNA methyltransferase 1, mitochondrial (MRM1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human RRNA methyltransferase 1, mitochondrial (MRM1) ELISA Kit employs a two-site sandwich ELISA to quantitate MRM1 in samples. An antibody specific for MRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MRM1; FLJ22578; mitochondrial rRNA methyltransferase 1 homolog

Product Properties

Kit components • Human RRNA methyltransferase 1, mitochondrial microplate
• Human RRNA methyltransferase 1, mitochondrial standard
• Human RRNA methyltransferase 1, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human RRNA methyltransferase 1, mitochondrial (MRM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRM1. No significant cross-reactivity or interference between Human MRM1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MRM1 (Mitochondrial RRNA Methyltransferase 1) is a Protein Coding gene. Among its related pathways are rRNA processing in the mitochondrion and rRNA processing in the nucleus and cytosol. GO annotations related to this gene include poly(A) RNA binding and methyltransferase activity. S-adenosyl-L-methionine-dependent 2-O-ribose methyltransferase that catalyzes the formation of 2-O-methylguanosine at position 1145 (Gm1145) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA.
Gene ID 79922
Alternative MRM1; FLJ22578; mitochondrial rRNA methyltransferase 1 homolog
Accession Q6IN84

Image & description

Fig.1. Human RRNA methyltransferase 1, mitochondrial (MRM1) Standard Curve.

Fig.1. Human RRNA methyltransferase 1, mitochondrial (MRM1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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