| Product name | Human RNA-binding protein 38 (RBM38) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human RNA-binding protein 38 (RBM38) ELISA Kit employs a two-site sandwich ELISA to quantitate RBM38 in samples. An antibody specific for RBM38 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBM38 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM38 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM38 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | RBM38; HSRNASEB; RNPC1; SEB4B; SEB4D; dJ800J21.2; CLL-associated antigen KW-5; RNA-binding region (RNP1; RRM) containing 1; RNA-binding region containing protein 1; ssDNA binding protein SEB4 |
| Kit components | • Human RNA-binding protein 38 microplate • Human RNA-binding protein 38 standard • Human RNA-binding protein 38 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human RNA-binding protein 38 (RBM38) ELISA Kit has high sensitivity and excellent specificity for detection of Human RBM38. No significant cross-reactivity or interference between Human RBM38 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Using microarray analysis to identify potential p53 (TP53) target genes in human cell lines, Shu et al. (2006) identified and cloned 2 variants of RBM38, which they called RNPC1a and RNPC1b. The deduced proteins contain 239 and 121 amino acids, respectively, and both contain an identical N-terminal canonical RNA recognition motif made up of 2 conserved submotifs, RNP1 and RNP2. Immunofluorescence analysis localized both proteins in the perinuclear membrane and cytosol, with a larger proportion of RNPC1b localized to the cytosol than the nucleus.Both RNPC1a and RNPC1b were also induced by several p63 (TP63) and p73 (TP73) isoforms. RNPC1 expression was induced by DNA-damaging agents in cells expressing wildtype p53, but not in cells lacking p53 expression. |
| Gene ID | 55544 |
| Alternative | RBM38; HSRNASEB; RNPC1; SEB4B; SEB4D; dJ800J21.2; CLL-associated antigen KW-5; RNA-binding region (RNP1; RRM) containing 1; RNA-binding region containing protein 1; ssDNA binding protein SEB4 |
| Accession | Q9H0Z9 |
Fig.1. Human RNA-binding protein 38 (RBM38) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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