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Human RNA-binding protein 24 (RBM24) ELISA Kit

Human RNA-binding protein 24 (RBM24) ELISA Kit

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Specification

Product name Human RNA-binding protein 24 (RBM24) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human RNA-binding protein 24 (RBM24) ELISA Kit employs a two-site sandwich ELISA to quantitate RBM24 in samples. An antibody specific for RBM24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBM24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RBM24; FLJ26355; FLJ30829; FLJ37697; RNPC6; dJ259A10.1; RNA-binding region (RNP1; RRM) containing 6

Product Properties

Kit components • Human RNA-binding protein 24 microplate
• Human RNA-binding protein 24 standard
• Human RNA-binding protein 24 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human RNA-binding protein 24 (RBM24) ELISA Kit has high sensitivity and excellent specificity for detection of Human RBM24. No significant cross-reactivity or interference between Human RBM24 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RBM24 is restricted to the lateral and ventral mesoderm during gastrulation and then localized to the somitic mesoderm, in a similar pattern as XMyoD gene. A 0.65kb X. tropicalis RBM24 regulatory region contains multiple E boxes (CANNTG), which are potential binding sites for MyoD and other bHLH proteins. Knockdown of Seb4 produces similar effects as those obtained by the dominant negative MyoD mutant, indicating that it is required for the expression of myogenic genes and myogenesis in the embryo. In cultured ectodermal explants, although overexpression of Seb4 has no obvious effect on myogenesis, knockdown of Seb4 inhibits the expression of myogenic genes and myogenesis induced by MyoD. Seb4/RBM24 is a direct target of MyoD and is required for myogenesis during Xenopus early development.
Gene ID 221662
Alternative RBM24; FLJ26355; FLJ30829; FLJ37697; RNPC6; dJ259A10.1; RNA-binding region (RNP1; RRM) containing 6
Accession Q9BX46

Image & description

Fig.1. Human RNA-binding protein 24 (RBM24) Standard Curve.

Fig.1. Human RNA-binding protein 24 (RBM24) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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