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Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit

Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit

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Specification

Product name Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit employs a two-site sandwich ELISA to quantitate RGS19 in samples. An antibody specific for RGS19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGS19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RGS19; GAIP; RGSGAIP; G alpha interacting protein; G protein signalling regulator 19; guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein; regulator of G-protein sig

Product Properties

Kit components • Human Regulator of G-protein signaling 19 microplate
• Human Regulator of G-protein signaling 19 standard
• Human Regulator of G-protein signaling 19 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Regulator of G-protein signaling 19 (RGS19) ELISA Kit has high sensitivity and excellent specificity for detection of Human RGS19. No significant cross-reactivity or interference between Human RGS19 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Sequence analysis predicted that GAIP is a 24.6-kD, hydrophilic protein that lacks a transmembrane domain and contains multiple potential phosphorylation sites. GAIP shares approximately 31% amino acid identity with regulator of G protein signaling-1 (RGS1) and -2 (RGS2); the 125-amino acid core domains of GAIP, RGS1, and RGS2 share approximately 64% homology. Northern blot analysis detected a 1.6-kb GAIP transcript in lung, placenta, liver, heart, and pancreas, with almost no expression detected in brain, skeletal muscle, and kidney. Yeast 2-hybrid binding analyses showed that through its core domain, GAIP interacts strongly with GNAI3 and weakly with GNAI2, but does not interact with GNA11.
Gene ID 10287
Alternative RGS19; GAIP; RGSGAIP; G alpha interacting protein; G protein signalling regulator 19; guanine nucleotide binding protein alpha inhibiting activity polypeptide 3 interacting protein; regulator of G-protein sig
Accession P49795

Image & description

Fig.1. Human Regulator of G-protein signaling 19 (RGS19) Standard Curve.

Fig.1. Human Regulator of G-protein signaling 19 (RGS19) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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