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Human Regulator of G-protein signaling 1 (RGS1) ELISA Kit

Human Regulator of G-protein signaling 1 (RGS1) ELISA Kit

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Specification

Product name Human Regulator of G-protein signaling 1 (RGS1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Regulator of G-protein signaling 1 (RGS1) ELISA Kit employs a two-site sandwich ELISA to quantitate RGS1 in samples. An antibody specific for RGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RGS1; 1R20; BL34; IER1; IR20; B-cell activation protein BL34; early response protein 1R20; immediate-early response 1; B-cell specific; regulator of G-protein signalling 1

Product Properties

Kit components • Human Regulator of G-protein signaling 1 microplate
• Human Regulator of G-protein signaling 1 standard
• Human Regulator of G-protein signaling 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Regulator of G-protein signaling 1 (RGS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RGS1. No significant cross-reactivity or interference between Human RGS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Regulator of G-protein signaling 1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.B-cell specific. Expression is relatively low in B-cells and chronic lymphocytic leukemia B-cells; however, in other types of malignant B-cell such as non-Hodgkin's lymphoma and hairy cell leukemia, expression is constitutively high.
Gene ID 5996
Alternative RGS1; 1R20; BL34; IER1; IR20; B-cell activation protein BL34; early response protein 1R20; immediate-early response 1; B-cell specific; regulator of G-protein signalling 1
Accession Q08116

Image & description

Fig.1. Human Regulator of G-protein signaling 1 (RGS1) Standard Curve.

Fig.1. Human Regulator of G-protein signaling 1 (RGS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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