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Human Receptor-type tyrosine-protein phosphatase gamma (PTPRG) ELISA Kit

Human Receptor-type tyrosine-protein phosphatase gamma (PTPRG) ELISA Kit

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Specification

Product name Human Receptor-type tyrosine-protein phosphatase gamma (PTPRG) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Receptor-type tyrosine-protein phosphatase gamma (PTPRG) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPRG in samples. An antibody specific for PTPRG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTPRG; HPTPG; PTPG; R-PTP-GAMMA; RPTPG; H_RG317H01.1; protein tyrosine phosphatase gamma; protein tyrosine phosphatase; receptor type; gamma polypeptide; receptor type protein tyrosine phosphatase gamma; recep

Product Properties

Kit components • Human Receptor-type tyrosine-protein phosphatase gamma microplate
• Human Receptor-type tyrosine-protein phosphatase gamma standard
• Human Receptor-type tyrosine-protein phosphatase gamma detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Receptor-type tyrosine-protein phosphatase gamma (PTPRG) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTPRG. No significant cross-reactivity or interference between Human PTPRG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Receptor-type tyrosine-protein phosphatase gamma is member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this PTP contains a carbonic anhydrase-like (CAH) domain, which is also found in the extracellular region of PTPRBETA/ZETA. This gene is located in a chromosomal region that is frequently deleted in renal cell carcinoma and lung carcinoma, thus is thought to be a candidate tumor suppressor gene.
Alternative PTPRG; HPTPG; PTPG; R-PTP-GAMMA; RPTPG; H_RG317H01.1; protein tyrosine phosphatase gamma; protein tyrosine phosphatase; receptor type; gamma polypeptide; receptor type protein tyrosine phosphatase gamma; recep
Accession Q98936

Image & description

Fig.1. Human Receptor-type tyrosine-protein phosphatase gamma (PTPRG) Standard Curve.

Fig.1. Human Receptor-type tyrosine-protein phosphatase gamma (PTPRG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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