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Human Ras-related protein Rab-3B (RAB3B) ELISA Kit

Human Ras-related protein Rab-3B (RAB3B) ELISA Kit

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Product name Human Ras-related protein Rab-3B (RAB3B) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Ras-related protein Rab-3B (RAB3B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB3B in samples. An antibody specific for RAB3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RAB3B; Brain antigen RAB3B

Product Properties

Kit components • Human Ras-related protein Rab-3B microplate
• Human Ras-related protein Rab-3B standard
• Human Ras-related protein Rab-3B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Ras-related protein Rab-3B (RAB3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAB3B. No significant cross-reactivity or interference between Human RAB3B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The mammalian RAB proteins show striking similarities to the S. cerevisiae YPT1 and SEC4 proteins, Ras-related GTP-binding proteins involved in the regulation of secretion.RAB3B protein shares 78% identity with RAB3A. After endocytosis, the PIGR-dIgA complex traverses several endosomal compartments and is exocytosed at the apical plasma membrane Rab3b localized to vesicular structures containing Pigr in canine kidney cells and that GTP-bound Rab3b interacted directly with the cytoplasmic domain of Pigr. Binding of dIgA to Pigr dissociated Rab3b, but a constitutively active Rab3b mutant maintained its interaction with Pigr in dIgA-treated cells. Furthermore, GTP-locked Rab3b inhibited dIgA-stimulated transcytosis.
Gene ID 5865
Alternative RAB3B; Brain antigen RAB3B
Accession P20337

Image & description

Fig.1. Human Ras-related protein Rab-3B (RAB3B) Standard Curve.

Fig.1. Human Ras-related protein Rab-3B (RAB3B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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