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Human RAC-gamma serine/threonine-protein kinase (AKT3) ELISA Kit

Human RAC-gamma serine/threonine-protein kinase (AKT3) ELISA Kit

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Specification

Product name Human RAC-gamma serine/threonine-protein kinase (AKT3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human RAC-gamma serine/threonine-protein kinase (AKT3) ELISA Kit employs a two-site sandwich ELISA to quantitate AKT3 in samples. An antibody specific for AKT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAKT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative AKT3; DKFZp434N0250; PKB-GAMMA; PKBG; PRKBG; RAC-PK-gamma; RAC-gamma; STK-2; AKT3 kinase; OTTHUMP00000037911; OTTHUMP00000037912; RAC-gamma serine/threonine protein kinase; protein kinase B gamma; serine threo

Product Properties

Kit components • Human RAC-gamma serine/threonine-protein kinase microplate
• Human RAC-gamma serine/threonine-protein kinase standard
• Human RAC-gamma serine/threonine-protein kinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human RAC-gamma serine/threonine-protein kinase (AKT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human AKT3. No significant cross-reactivity or interference between Human AKT3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.Mice lacking Akt3 have a normal glucose metabolism (no diabetes), have approximately normal body weight, but have a 25% reduction in brain mass. Incidentally, Akt3 is highly expressed in the brain.
Gene ID 10000
Alternative AKT3; DKFZp434N0250; PKB-GAMMA; PKBG; PRKBG; RAC-PK-gamma; RAC-gamma; STK-2; AKT3 kinase; OTTHUMP00000037911; OTTHUMP00000037912; RAC-gamma serine/threonine protein kinase; protein kinase B gamma; serine threo
Accession Q9Y243

Image & description

Fig.1. Human RAC-gamma serine/threonine-protein kinase (AKT3) Standard Curve.

Fig.1. Human RAC-gamma serine/threonine-protein kinase (AKT3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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