|Product name||Human Putative platelet basic protein-like 2 (PPBPL2) ELISA Kit|
|Applications notes||This Human Putative platelet basic protein-like 2 (PPBPL2) ELISA Kit employs a two-site sandwich ELISA to quantitate PPBPL2 in samples. An antibody specific for PPBPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPBPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPBPL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPBPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Putative platelet basic protein-like 2 microplate
• Human Putative platelet basic protein-like 2 standard
• Human Putative platelet basic protein-like 2 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Putative platelet basic protein-like 2 (PPBPL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PPBPL2. No significant cross-reactivity or interference between Human PPBPL2 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||By screening an adult human lung cDNA library, Luzi and Strayer (1995) identified PPBPL2, which they called A2. The deduced protein contains 106 amino acids. By gel retardation assay, Luzi and Strayer (1995) demonstrated that PPBPL2 exhibited binding activity to a 212-bp region of the SPB (SFTPB) promoter and slightly increased transcription of a luciferase reporter containing the promoter region. Beta-thromboglobulin (β-thromboglobulin, also called Pro-Platelet basic protein) is a protein that is stored in alpha-granules of platelets and released in large amounts after platelet activation.It is a chemoattractant, strongly for fibroblasts and weakly for neutrophils. It is a stimulator of mitogenesis, extracellular matrix synthesis, glucose metabolism, and plasminogen activator synthesis in human fibroblasts.|
Fig.1. Human Putative platelet basic protein-like 2 (PPBPL2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.