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Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit

Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit

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Specification

Product name Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT1 in samples. An antibody specific for WNT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative WNT1; INT1; Wingless-type MMTV integration site family; member 1 (oncogene INT1)

Product Properties

Kit components • Human Proto-oncogene Wnt-1 microplate
• Human Proto-oncogene Wnt-1 standard
• Human Proto-oncogene Wnt-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human WNT1. No significant cross-reactivity or interference between Human WNT1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The WNT gene family consists of structurally related genes which encode secreted signaling proteins. WNT1 is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
Gene ID 7471
Alternative WNT1; INT1; Wingless-type MMTV integration site family; member 1 (oncogene INT1)
Accession P04628

Image & description

Fig.1. Human Proto-oncogene Wnt-1 (WNT1) Standard Curve.

Fig.1. Human Proto-oncogene Wnt-1 (WNT1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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