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Human Protein-tyrosine kinase 6 (PTK6) ELISA Kit

Human Protein-tyrosine kinase 6 (PTK6) ELISA Kit

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Specification

Product name Human Protein-tyrosine kinase 6 (PTK6) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Protein-tyrosine kinase 6 (PTK6) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK6 in samples. An antibody specific for PTK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTK6; BRK; FLJ42088; breast tumor kinase; protein-tyrosine kinase BRK

Product Properties

Kit components • Human Protein-tyrosine kinase 6 microplate
• Human Protein-tyrosine kinase 6 standard
• Human Protein-tyrosine kinase 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Protein-tyrosine kinase 6 (PTK6) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTK6. No significant cross-reactivity or interference between Human PTK6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tyrosine-protein kinase 6 is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. The deduced 451-amino acid polypeptide sequence was composed of 3 domains: an SH3 domain, an SH2 domain, and a catalytic domain. The sequence of BRK, unlike that of SRC, does not include an N-terminal myristoylation domain.
Gene ID 5753
Alternative PTK6; BRK; FLJ42088; breast tumor kinase; protein-tyrosine kinase BRK
Accession Q13882

Image & description

Fig.1. Human Protein-tyrosine kinase 6 (PTK6) Standard Curve.

Fig.1. Human Protein-tyrosine kinase 6 (PTK6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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