Product Information

• Product name

  Human Protein LYRIC (MTDH) ELISA Kit

• Reactivity

  Human

• Applications

  ELISA

• Application notes

  This Human Protein LYRIC (MTDH) ELISA Kit employs a two-site sandwich ELISA to quantitate MTDH in samples. An antibody specific for MTDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTDH bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Detection method

  Colorimetric

• Sample type

  Cell culture supernatants, Other biological fluids, Plasma, Serum

• Assay type

  Sandwich ELISA (quantitative)

• Assay duration

  Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Human Protein LYRIC (MTDH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Product Properties

• Kit components

  • Human Protein LYRIC microplate
  • Human Protein LYRIC standard
  • Human Protein LYRIC detect antibody
  • Streptavidin-HRP
  • Standard diluent
  • Assay buffer
  • HRP substrate
  • Stop solution
  • Wash buffer
  • Plate covers

• Features & Benefits

  Human Protein LYRIC (MTDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTDH. No significant cross-reactivity or interference between Human MTDH and analogues was observed.

• Calibration range

  Please inquire

• Limit of detection

  Please inquire

• Usage notes

  • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
  • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
  • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
  • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
  • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
  • It is recommended that all samples and standards be assayed in duplicate or triplicate.

• Storage instructions

  The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  MTDH (Metadherin) is a Protein Coding gene. Diseases associated with MTDH include Nervous System Disease. Among its related pathways are Adhesion and Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases. GO annotations related to this gene include poly(A) RNA binding and double-stranded RNA binding. Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

• Gene ID

  92140

• Alternative names

  MTDH; 3D3; AEG-1; AEG1; LYRIC; LYRIC/3D3; astrocyte elevated gene-1; metastasis adhesion protein

• Accession

  Q86UE4